GENOTYPING OF HERPES-SIMPLEX VIRUSES BY POLYMERASE CHAIN-REACTION

被引：40
作者：
PIIPARINEN, H
VAHERI, A
机构：
[1] Department of Virology, University of Helsinki, Helsinki
来源：
ARCHIVES OF VIROLOGY | 1991年 / 119卷 / 3-4期
关键词：
D O I：
10.1007/BF01310676
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detection and genotyping of herpes simplex viruses (HSV) from virus isolates. It was possible to detect and type HSV using only one primer pair from the HSV DNA polymerase coding genes. After PCR the type of HSV present was determined on the basis of size of the ethidiumbromide-stained band after agarose gel electrophoresis. A hybridization test with radioactive target oligonucleotide probes after PCR was also developed to confirm the typing result.
引用
收藏
页码：275 / 283
页数：9
相关论文
共 34 条
[1]   RAPID DIAGNOSIS OF HERPES-SIMPLEX ENCEPHALITIS BY NESTED POLYMERASE CHAIN-REACTION ASSAY OF CEREBROSPINAL-FLUID [J].
AURELIUS, E ;
JOHANSSON, B ;
SKOLDENBERG, B ;
STALAND, A ;
FORSGREN, M .
LANCET, 1991, 337 (8735) :189-192
[2]   IDENTIFICATION AND TYPING OF HERPES-SIMPLEX VIRUSES WITH MONOCLONAL-ANTIBODIES [J].
BALACHANDRAN, N ;
FRAME, B ;
CHERNESKY, M ;
KRAISELBURD, E ;
KOURI, Y ;
GARCIA, D ;
LAVERY, C ;
RAWLS, WE .
JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 16 (01) :205-208
[3]   HERPES-SIMPLEX KERATITIS [J].
BINDER, PS .
SURVEY OF OPHTHALMOLOGY, 1977, 21 (04) :313-331
[4]   RESTRICTION ENDONUCLEASE FINGERPRINTING OF HERPES-SIMPLEX VIRUS-DNA - NOVEL EPIDEMIOLOGICAL TOOL APPLIED TO A NOSOCOMIAL OUTBREAK [J].
BUCHMAN, TG ;
ROIZMAN, B ;
ADAMS, G ;
STOVER, BH .
JOURNAL OF INFECTIOUS DISEASES, 1978, 138 (04) :488-498
[5]  
COREY L, 1988, LANCET, V1, P1
[6]  
CRAIG CP, 1988, J INFECT DIS, V127, P365
[7]   POLYMERASE CHAIN-REACTION FOR FAST, NONRADIOACTIVE DETECTION OF HIGH-RISK AND LOW-RISK PAPILLOMAVIRUS TYPES IN ROUTINE CERVICAL SPECIMENS AND IN BIOPSIES [J].
DALLAS, PB ;
FLANAGAN, JL ;
NIGHTINGALE, BN ;
MORRIS, BJ .
JOURNAL OF MEDICAL VIROLOGY, 1989, 27 (02) :105-111
[8]  
Davis L. G., 1986, BASIC METHODS MOL BI
[9]  
DOWDLE WR, 1967, J IMMUNOL, V99, P974
[10]   MONOCLONAL-ANTIBODIES TO HERPES-SIMPLEX VIRUSES - USE IN ANTIGENIC TYPING AND RAPID DIAGNOSIS [J].
GOLDSTEIN, LC ;
COREY, L ;
MCDOUGALL, JK ;
TOLENTINO, E ;
NOWINSKI, RC .
JOURNAL OF INFECTIOUS DISEASES, 1983, 147 (05) :829-837
1234