Streptozotocin-Induced Adaptive Modification of Mitochondrial Supercomplexes in Liver of Wistar Rats and the Protective Effect of Moringa oleifera Lam

被引：17
作者：
Alejandra Sanchez-Munoz, Maria [1 ]
Andrea Valdez-Solana, Monica [1 ,2 ]
Ibeth Campos-Almazan, Mara
Flores-Herrera, Oscar [3 ]
Esparza-Perusquia, Mercedes [3 ]
Olvera-Sanchez, Sofia [3 ]
Garcia-Arenas, Guadalupe [4 ]
Avitia-Dominguez, Claudia [2 ]
Tellez-Valencia, Alfredo [2 ]
Sierra-Campos, Erick [1 ]
机构：
[1] Univ Juarez Estado Durango Campus, Fac Ciencias Quim, Gomez Palacio, Dgo, Mexico
[2] Univ Juarez Estado Durango Campus, Fac Med & Nutr, Gomez Palacio, Dgo, Mexico
[3] Univ Nacl Autonoma Mexico, Fac Med, Dept Bioquim, Mexico City, DF, Mexico
[4] Univ Juarez Estado Durango Campus, Fac Ciencias Salud, Gomez Palacio, Dgo, Mexico
来源：
BIOCHEMISTRY RESEARCH INTERNATIONAL | 2018年 / 2018卷
关键词：
D O I：
10.1155/2018/5681081
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The increasing prevalence of diabetes continues to be a major health issue worldwide. Alteration of mitochondrial electron transport chain is a recognized hallmark of the diabetic-associated decline in liver bioenergetics; however, the molecular events involved are only poorly understood. Moringa oleifera is used for the treatment of diabetes. However, its role on mitochondrial functionality is not yet established. This study was aimed to evaluate the effect of M. oleifera extract on supercomplex formation, ATPase activity, ROS production, GSH levels, lipid peroxidation, and protein carbonylation. The levels of lipid peroxidation and protein carbonylation were increased in diabetic group. However, the levels were decreased in Moringa-treated diabetic rats. Analysis of in-gel activity showed an increase in all complex activities in the diabetic group, but spectrophotometric determinations of complex II and IV activities were unaffected in this treatment. However, we found an oxygen consumption abolition through complex I-III-IV pathway in the diabetic group treated with Moringa. While respiration with succinate feeding into complex II-III-IV was increased in the diabetic group. These findings suggest that hyperglycemia modifies oxygen consumption, supercomplexes formation, and increases ROS levels in mitochondria from the liver of STZ-diabetic rats, whereas M. oleifera may have a protective role against some alterations.
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页数：15
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