STUDIES ON LUMBO VIRUS-REPLICATION .2. PROPERTIES OF VIRAL RIBONUCLEOPROTEINS AND CHARACTERIZATION OF MESSENGER-RNAS

Ribonucleoprotein-like structures were isolated from Lumbo virus-infected BHK21 [baby hamster kidney] cells. Like the viral ribonucleoproteins, they belonged to 3 classes sedimenting at 105, 85 and 45 S, all of which banded at a density of 1.34 g/cm3 in CsCl gradients. A major polypeptide of MW 25,000 was associated with these structures. Both negative- and positive-stranded RNPs were detected. Lumbo virus mRNAs isolated from infected cell polysomes were shown by annealing experiments to contain sequences complementary to those found in the viral genome.