OLIGONUCLEOTIDE PROBES FOR BORDETELLA-BRONCHISEPTICA BASED ON 16S RIBOSOMAL-RNA SEQUENCES

被引：2

作者：

TANEDA, A

FUTO, S

MITSUSE, S

SETO, Y

OKADA, M

SAKANO, T

机构：

[1] NIPPON FLOURMILLS CO LTD,CENT LAB,ATSUGI,KANAGAWA,JAPAN

[2] GIFU UNIV,UNITED GRAD SCH AGR SCI,GIFU,JAPAN

来源：

VETERINARY MICROBIOLOGY | 1994年 / 42卷 / 04期

关键词：

BORDETELLA BRONCHISEPTICA; GENETICS; DNA PROBE;

D O I：

10.1016/0378-1135(94)90061-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bordetella bronchiseptica 16S ribosomal RNA (rRNA) gene was cloned and identified. On the basis of information from computer-assisted sequence comparison of the B. bronchiseptica 16S RRNA sequences with that of other bacterial species, we constructed B. bronchiseptica-specific oligonucleotide probes complementary to variable regions in the 16S rRNA molecule. Specificity of these P-32-labeled oligo-nucleotide probes was tested in a RNA/DNA hybridization with B. bronchiseptica strains and other bacterial strains. Probe BB4 was more specific than three other oligonucleotide probes. This probe BB4 was Sensitive enough to be able to detect 10(4) bacterial cells.

引用

页码：297 / 305

页数：9

共 8 条

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