ROLE OF GASTRIC PH IN ISOLATION OF HELICOBACTER-MUSTELAE FROM THE FECES OF FERRETS

被引：51

作者：

FOX, JG ^{[1
]}

BLANCO, MC ^{[1
]}

YAN, L ^{[1
]}

SHAMES, B ^{[1
]}

POLIDORO, D ^{[1
]}

DEWHIRST, FE ^{[1
]}

PASTER, BJ ^{[1
]}

机构：

[1] FORSYTH DENT CTR,BOSTON,MA 02115

来源：

GASTROENTEROLOGY | 1993年 / 104卷 / 01期

关键词：

D O I：

10.1016/0016-5085(93)90839-5

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: Helicobacter mustelae colonizes the gastric mucosa of ferrets and causes persistent chronic gastritis. Methods: Hypochlorhydria, as measured by gastric pH probe, was induced by administering oral omeprazole, a proton pump inhibitor of the parietal cell, to adult ferrets in two separate experiments. Feces of ferrets were cultured for H. mustelae before, during, and after omeprazole therapy. Results: H. mustelae was isolated in 23 of 55 (41.8%) sequential fecal samples collected during omeprazole therapy. The same ferrets with acidic gastric pH had H. mustelae isolated in 6 of 62 (9.7%) of the fecal cultures (P < 0.01). A DNA species-specific H. mustelae probe confirmed the presence of the organism in both the stomach and feces of all 5 ferrets. In 4 of 5 ferrets restriction enzyme patterns of the gastric H. mustelae were identical to those of the fecal H. mustelae strains. Conclusions: Hypochlorhydria promotes fecal transmission of a gastric Helicobacter organism. The H. mustelae-colonized ferret provides an ideal model to study the epidemiology and pathogenesis of Helicobacter pylori-induced gastritis. © 1993.

引用

页码：86 / 92

页数：7

共 34 条

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