AMINO ACIDS CONTENTS IN BARLEY AND WHEAT

In this investigation of 2 barley, 2 durum and 2 aestivum wheat divergent cultivars were identified presence of amino acids by using method of chromatography. The method of spectrophotometry were used for establishing concentration of identified amino acids. Total concentration of free amino acids was determined by standard curved line for tyrozine, while concentration for each amino acid was determined by as well method by standard curved line for glycine. Qualitative and quantitative analysis of amino acids showed that glutamic acid, proline, threonine, norvaline, tryptophan, were the most present in seeds of analyzed cereal species cultivars. Analysis of quantity of free amino acid indicated to their high concentration (near the 100 mg/ml) in durum wheat YG-5251, YG-9052, and in barley cultivars Novosadski 703 and Novosadski 309 (over 80 mg/ml). Concentration of glutamic acid in durum wheat was 4.5 mg/ml and aestivum wheat 5.5 mg/ml and was higher than contents of other amino acids. In barley cultivars the highest concentration was established for amino acids proline (31.0 mg/ml) and threonine (44.2 mg/ml).