PLASMODIUM-VIVAX MEROZOITE SURFACE PROTEIN-1 C-TERMINAL RECOMBINANT PROTEINS IN BACULOVIRUS

被引：37

作者：

LONGACRE, S ^{[1
]}

MENDIS, KN ^{[1
]}

DAVID, PH ^{[1
]}

机构：

[1] UNIV COLOMBO, FAC MED, DEPT PARASITOL, COLOMBO, SRI LANKA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1994年 / 64卷 / 02期

关键词：

MALARIA; PLASMODIUM VIVA; MEROZOITE SURFACE PROTEIN 1 (MSP1); BACULOVIRUS; GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR;

D O I：

10.1016/0166-6851(94)00002-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant proteins derived from the Plasmodium vivax merozoite surface protein 1 have been produced in the baculovirus expression system. These proteins correspond approximately to the Plasmodium vivax analogs of the 42-kDa or 19-kDa C-terminal processing products previously described for Plasmodium falciparum. Each was produced in two versions, either as a membrane-bound entity located on the cell surface and probably carrying a glycosylphosphatidylinositol addition, or as a secreted entity lacking a membrane anchor. Many native conformational epitopes appear to be accurately reproduced in these molecules. Both the 42-kDa and 19-kDa analogs an be N-glycosylated in the baculovirus system and the N-glycosylation appears to be necessary for efficient secretion of both the 42-kDa and 19-kDa recombinant proteins.

引用

页码：191 / 205

页数：15

共 45 条

[1] PLASMODIUM-VIVAX - EXOERYTHROCYTIC SCHIZONTS RECOGNIZED BY MONOCLONAL-ANTIBODIES AGAINST BLOOD-STAGE SCHIZONTS [J].

ALEY, SB ;

BARNWELL, JW ;

BATES, MD ;

COLLINS, WE ;

HOLLINGDALE, MR .

EXPERIMENTAL PARASITOLOGY, 1987, 64 (02) :188-194

[2] REACTION OF MONOCLONAL-ANTIBODIES WITH PLASMA-MEMBRANE PROTEINS AFTER BINDING ON NITROCELLULOSE - RENATURATION OF ANTIGENIC SITES AND REDUCTION OF NONSPECIFIC ANTIBODY-BINDING [J].

BIRK, HW ;

KOEPSELL, H .

ANALYTICAL BIOCHEMISTRY, 1987, 164 (01) :12-22

[3] SECONDARY PROCESSING OF THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE PROTEIN-1 (MSP1) BY A CALCIUM-DEPENDENT MEMBRANE-BOUND SERINE PROTEASE - SHEDDING OF MSP133 AS A NONCOVALENTLY ASSOCIATED COMPLEX WITH OTHER FRAGMENTS OF THE MSP1 [J].

BLACKMAN, MJ ;

HOLDER, AA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 50 (02) :307-316

[4] PROTEOLYTIC PROCESSING OF THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE PROTEIN-1 PRODUCES A MEMBRANE-BOUND FRAGMENT CONTAINING 2 EPIDERMAL GROWTH FACTOR-LIKE DOMAINS [J].

BLACKMAN, MJ ;

LING, IT ;

NICHOLLS, SC ;

HOLDER, AA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (01) :29-34

[5] A SINGLE FRAGMENT OF A MALARIA MEROZOITE SURFACE PROTEIN REMAINS ON THE PARASITE DURING RED-CELL INVASION AND IS THE TARGET OF INVASION-INHIBITING ANTIBODIES [J].

BLACKMAN, MJ ;

HEIDRICH, HG ;

DONACHIE, S ;

MCBRIDE, JS ;

HOLDER, AA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (01) :379-382

[6] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].

BONNER, WM ;

LASKEY, RA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88

[7] GLYCOLIPID ANCHORAGE OF PLASMODIUM-FALCIPARUM SURFACE-ANTIGENS [J].

BRETON, CB ;

ROSENBERRY, TL ;

DASILVA, LHP .

RESEARCH IN IMMUNOLOGY, 1990, 141 (08) :743-755

[8]

BURNS JM, 1989, J IMMUNOL, V143, P2670

[9]

CHANG SP, 1992, J IMMUNOL, V149, P548

[10] MAPPING OF THE REGION PREDOMINANTLY RECOGNIZED BY ANTIBODIES TO THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE-ANTIGEN MSA-1 [J].

COOPER, JA ;

COOPER, LT ;

SAUL, AJ .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 51 (02) :301-312

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