H-PHOSPHONATE OLIGONUCLEOTIDE SYNTHESIS ON A POLYETHYLENE-GLYCOL POLYSTYRENE COPOLYMER

被引：38

作者：

GAO, HT ^{[1
]}

GAFFNEY, BL ^{[1
]}

JONES, RA ^{[1
]}

机构：

[1] RUTGERS STATE UNIV,DEPT CHEM,PISCATAWAY,NJ 08855

来源：

TETRAHEDRON LETTERS | 1991年 / 32卷 / 40期

基金：

美国国家卫生研究院;

关键词：

OLIGONUCLEOTIDE SYNTHESIS; H-PHOSPHONATE; AUTOMATED TRITYL ASSAY;

D O I：

10.1016/0040-4039(91)80062-B

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The scale of oligonucleotide synthesis can be increased by a factor of 2-3 by using a polyethylene glycol/polystyrene copolymer support in place of controlled-pore glass. This support allows synthesis on a scale of 30-mu-mole in a standard 10-15-mu-mole cartridge. In addition, the recovery and reuse of H-phosphonate monomers are reported and a system for automation of the trityl assay is described.

引用

页码：5477 / 5480

页数：4

共 8 条

[1] POLYSTYRENE-SUPPORTED SYNTHESIS BY THE PHOSPHITE TRIESTER APPROACH - AN ALTERNATIVE FOR THE LARGE-SCALE SYNTHESIS OF SMALL OLIGODEOXYRIBONUCLEOTIDES [J].