MODIFIED DEOXYOLIGONUCLEOTIDES STABLE TO EXONUCLEASE DEGRADATION IN SERUM

被引：346

作者：

SHAW, JP ^{[1
]}

KENT, K ^{[1
]}

BIRD, J ^{[1
]}

FISHBACK, J ^{[1
]}

FROEHLER, B ^{[1
]}

机构：

[1] GILEAD SCI,346 LAKESIDE DR,FOSTER CITY,CA 94404

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 04期

关键词：

D O I：

10.1093/nar/19.4.747

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding affinity for the complementary DNA sequence.

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页码：747 / 750

页数：4

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