ANALYTICAL AND MECHANISTIC ASPECTS OF THE ELECTROCHEMICAL OXIDATION OF KETO STEROIDS DERIVATIZED WITH PHENYLHYDRAZINE, (4-NITROPHENYL)HYDRAZINE, AND (2,4-DINITROPHENYL)HYDRAZINE

被引：17

作者：

BOND, AM ^{[1
]}

HOLLENKAMP, AF ^{[1
]}

THOMPSON, SB ^{[1
]}

BOURNE, AR ^{[1
]}

HUF, PA ^{[1
]}

WATSON, TG ^{[1
]}

机构：

[1] DEAKIN UNIV,DIV BIOL & HLTH SCI,GEELONG,VIC 3217,AUSTRALIA

来源：

ANALYTICAL CHEMISTRY | 1988年 / 60卷 / 10期

关键词：

BIOLOGICAL MATERIALS - Chromatographic Analysis - ELECTROCHEMISTRY - ELECTRODES - Materials - METHANOL;

D O I：

10.1021/ac00161a015

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrochemical oxidation of biologically important 3- and 17-keto steroids derivatized with phenylhydrazines has been investigated at platinum and glassy carbon electrodes in aqueous methanol. The oxidation process has been used in the amperometric detection of keto steroids in nonmammalian biological fluids after separation by reverse-phase liquid chromatography. The derivatization was shown to be quantitative, and the response linear with the detection limit of 1-5 ng for injection volumes of 20 mu L.

引用

页码：1023 / 1027

页数：5

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