COMPARISON OF POLYMERASE CHAIN-REACTION, DIRECT IMMUNOFLUORESCENCE, CELL-CULTURE AND ENZYME-IMMUNOASSAY FOR THE DETECTION OF CHLAMYDIA-PSITTACI IN BULL SEMEN

被引：24

作者：

DOMEIKA, M ^{[1
]}

GANUSAUSKAS, A ^{[1
]}

BASSIRI, M ^{[1
]}

FROMAN, G ^{[1
]}

MARDH, PA ^{[1
]}

机构：

[1] LITHUANIAN VET ACAD,CHLAMYDIOL LAB,KAUNAS,LITHUANIA

来源：

VETERINARY MICROBIOLOGY | 1994年 / 42卷 / 04期

关键词：

CHLAMYDIA PSITTACI; CATTLE CHLAMYDIA SP; DIAGNOSIS CHLAMYDIA SP; POLYMERASE CHAIN REACTION; DIRECT IMMUNOFLUORESCENCE TEST; CELL CULTURE TEST; ENZYME IMMUNOASSAY;

D O I：

10.1016/0378-1135(94)90058-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The polymerase chain reaction (PCR), direct immunofluorescence (DIF; JMAGEN Chlamydia, DAKO Diagnostics, UK), cell culture (CC) and enzyme immunoassay (ELA; Syva MicroTrak) were evaluated for detection of Chlamydia psittaci in bull semen. Three specimens were collected from each of 47 bulls at 3-6 month intervals (134 samples). Judging by the number of samples tested (n=134), PCR showed a sensitivity of 90.9%, DIF of 93.9%, CC of 72.7% and EIA of 81.8%. PCR, DIF, CC and EIA were 100% specific, respectively. Of the 47 bulls the maximum number of chlamydia-positive animals (n=14) was revealed when repeated tests were made by PCR. PCR detected 21.4% more positives than DIF and CC and 35.7% more than ELA. Although CC was less sensitive judging by the number of samples tested, it was as sensitive as DIF (78.6%) when judged by the number of bulls investigated. All bulls found to be chlamydia-positive remained so throughout the investigation, which lasted 18 months.

引用

页码：273 / 280

页数：8

共 30 条

[1]

BORYCZKO B, 1973, MED WETER, V29, P483

[2] MECHANISMS OF INFERTILITY IN GENITAL-TRACT INFECTIONS DUE TO CHLAMYDIA-PSITTACI TRANSMITTED THROUGH CONTAMINATED SEMEN [J].