SORBITOL DEHYDROGENASE REACTOR FOR FLUOROMETRIC DETECTION OF ALDITOLS IN A LIQUID-CHROMATOGRAPHIC SYSTEM

被引：6

作者：

KIBA, N

MATSUSHITA, R

OYAMA, Y

FURUSAWA, M

机构：

[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 248卷 / 02期

关键词：

LIQUID CHROMATOGRAPHY; FLUOROMETRY; ENZYME REACTOR; SORBITOL DEHYDROGENASE;

D O I：

10.1016/S0003-2670(00)84653-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Sorbitol dehydrogenase (E.C. 1.1.1.14) is immobilized chemically on poly(vinyl alcohol) beads and used as a column reactor in a liquid chromatographic system for the specific detection of D-xylitol, D-sorbitol and D-ribitol. The alditols are separated from each other on a TSK gel SCX (5-mu-m, Ca2+ form) column (30 cm x 7.8 mm i.d.) with water as eluent. The NADH produced from the enzymatic reaction is monitored spectrofluorimetrically. Calibration graphs are linear between 2 and 500-mu-M for xylitol, 5 and 800-mu-M for sorbitol and 2 and 500-mu-M for ribitol.

引用

页码：367 / 370

页数：4