ISLET-SPECIFIC T-CELL CLONES FROM THE NOD MOUSE RESPOND TO BETA-GRANULE ANTIGEN

被引：111

作者：

BERGMAN, B

HASKINS, K

机构：

[1] UNIV COLORADO,HLTH SCI CTR,BARBARA DAVIS CTR CHILDHOOD DIABET,DENVER,CO 80262

[2] UNIV COLORADO,HLTH SCI CTR,DEPT IMMUNOL,DENVER,CO 80262

来源：

DIABETES | 1994年 / 43卷 / 02期

关键词：

D O I：

10.2337/diabetes.43.2.197

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Islet-reactive T-cell clones from NOD mice provide an important approach to the investigation of antigens with relevance to type I diabetes. To identify a source of beta-cell antigen suitable for biochemical studies, we have used two islet-specific, diabetogenic T-cell clones to test beta-tumor cells. beta-tumor cell lines, maintained in continuous culture, were found to lose antigenicity rapidly. However, cells harvested directly from beta-tumors arising spontaneously in the transgenic NOD/Lt-Tg(RIPTag)1Lt mouse proved to be a potent source of beta-cell antigen for the T-cell clones. Subcellular fractionation of beta-tumor cells showed that the T-cell antigen was highly enriched in the beta-granule fraction and that this activity was associated with the granule membrane.

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页码：197 / 203

页数：7

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