EFFECT OF GROWTH TEMPERATURE ON MAINTENANCE OF VIRULENT RHODOCOCCUS-EQUI

被引：26

作者：

TAKAI, S ^{[1
]}

SUGAWARA, T ^{[1
]}

WATANABE, Y ^{[1
]}

SASAKI, Y ^{[1
]}

TSUBAKI, S ^{[1
]}

SEKIZAKI, T ^{[1
]}

机构：

[1] NATL INST ANIM HLTH,TSUKUBA,IBARAKI 305,JAPAN

来源：

VETERINARY MICROBIOLOGY | 1994年 / 39卷 / 1-2期

关键词：

D O I：

10.1016/0378-1135(94)90099-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Repeated passage of virulent Rhodococcus equi ATCC 33701 and L1 at 38 degrees C resulted in attenuation of the strains as a result of curing the virulence plasmid; at 30 degrees C, repeated passage had no such effect. At a temperature of 38 degrees C the plasmid-bearing cells replicated more slowly than their plasmid-cured derivatives and so were gradually replaced by cells lacking plasmids. In contrast, at a temperature of 30 degrees C the growth rate of either strain was not affected by the presence or absence of the plasmid. No plasmid-cured derivative was recovered from mouse organs at 48 h after inoculation of a mixture of equal numbers of bacteria with and without plasmids. It is concluded that under nonselective conditions growth temperature is an important factor in maintaining the virulence of R. equi.

引用

页码：187 / 192

页数：6

共 16 条

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