DETERMINATION OF AFLATOXINS IN AIRBORNE DUST FROM FEED FACTORIES BY AUTOMATED IMMUNOAFFINITY COLUMN CLEANUP AND LIQUID-CHROMATOGRAPHY

被引：25

作者：

KUSSAK, A ^{[1
]}

ANDERSSON, B ^{[1
]}

ANDERSSON, K ^{[1
]}

机构：

[1] NATL INST OCCUPAT HLTH,DIV ANALYT CHEM,S-90713 UMEA,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 708卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00365-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the determination of aflatoxins in airborne dust is described. The dust samples were extracted with acetone-water, cleaned up on immunoaffinity columns and analysed by HPLC, including a post-column derivatization reaction with bromine and fluorescence detection. The method was evaluated with filters spiked with 62-595 pg of aflatoxins B-1, B-2, G(1), and G(2), respectively. Average aflatoxin recoveries for B-1, B-2, G(1) and G(2) were 97, 101, 87 and 84%, respectively. The method was used for airborne dust collected at feed factories. The detection limit was 3.1 ng/g dust for aflatoxins B-1, G(1) and G(2) and 1.8 ng/g for aflatoxin B-2, for a 10-mg dust sample.

引用

页码：55 / 60

页数：6

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