PULSED FIELD SEQUENCING GEL-ELECTROPHORESIS

被引：20
作者：
BRASSARD, E [1 ]
TURMEL, C [1 ]
NOOLANDI, J [1 ]
机构：
[1] XEROX RES CTR CANADA LTD,2660 SPEAKMAN DR,MISSISSAUGA L5K 2L1,ONTARIO,CANADA
来源：
ELECTROPHORESIS | 1992年 / 13卷 / 08期
关键词：
D O I：
10.1002/elps.11501301109
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of pulsed fields on sequencing gel electrophoresis is investigated, using DNA fragment markers ranging in size from 20 to 6557 bases. For high continuous electric fields (5000 V/55 cm) band inversion is observed in which fragments larger than 4000 bases migrate faster than those of 800-1000 bases. The use of one-dimensional pulsed field gel electrophoresis (ODPFGE) eliminates band inversion and extends the monotonic size-mobility relationship of the DNA markers up to about 4000 bases. The relevance of these results, obtained using a manual sequencing process with autoradiographic detection, to automated sequencers is discussed.
引用
收藏
页码：529 / 535
页数：7
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