SYNTHESIS OF A RADIOACTIVE LABELED UNDECAGOLDCLUSTER FOR APPLICATION IN X-RAY STRUCTURE-ANALYSIS OF RIBOSOMES

被引：3

作者：

BOECKH, T

WITTMANN, HG

机构：

[1] Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1075卷 / 01期

关键词：

HEAVY ATOM; RIBOSOME; UNDECAGOLDCLUSTER;

D O I：

10.1016/0304-4165(91)90073-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to quantitate and to determine the extent of binding of a heavy atom derivative to ribosomal particles and other components of protein biosynthesis, we prepared a C-14-radioactive labeled monofunctional undecagoldcluster. This cluster can be bound either to exposed sulfhydryl groups at the surface of the ribosomes and its subunits, or to high nucleophilic amino groups of nucleic acids as well as to proteins.

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页码：50 / 55

页数：6

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