PURIFICATION AND PARTIAL CHARACTERIZATION OF GLYOXALASE-I FROM A HIGHER-PLANT BRASSICA-JUNCEA

被引：27

作者：

DESWAL, R ^{[1
]}

SOPORY, SK ^{[1
]}

机构：

[1] JAWAHARLAL NEHRU UNIV,SCH LIFE SCI,PLANT RES LAB,NEW DELHI 110067,INDIA

来源：

FEBS LETTERS | 1991年 / 282卷 / 02期

关键词：

GLYOXALASE-I; ENZYME PURIFICATION; METHYLGLYOXAL; GLUTATHIONE; S-HEXYL GLUTATHIONE;

D O I：

10.1016/0014-5793(91)80494-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The glyoxalase I was purified from Brassica juncea by affinity chromatography on S-hexyl GSH sepharose 4B. Homogeneity of the protein was confirmed electrophoretically by a silver stained gel. Activity staining on a native starch gel also showed a single band. The effect of glutathione, methylglyoxal, and pH on enzyme kinetics was studied. Magnesium was found to stimulate the enzyme activity.

引用

页码：277 / 280

页数：4

共 20 条

[1] CHARACTERIZATION OF GLYOXALASE-I PURIFIED FROM PIG ERYTHROCYTES BY AFFINITY CHROMATOGRAPHY [J].

ARONSSON, AC ;

MANNERVIK, B .

BIOCHEMICAL JOURNAL, 1977, 165 (03) :503-509

[2] PURIFICATION OF GLYOXALASE-I FROM HUMAN ERYTHROCYTES BY THE USE OF AFFINITY CHROMATOGRAPHY AND SEPARATION OF THE 3 ISOENZYMES [J].

ARONSSON, AC ;

TIBBELIN, G ;

MANNERVIK, B .

ANALYTICAL BIOCHEMISTRY, 1979, 92 (02) :390-393

[3] INHIBITION OF CELL-PROLIFERATION AND GLYOXALASE-I ACTIVITY BY CALMODULIN INHIBITORS AND LITHIUM IN BRASSICA-OLERACEA [J].

BAGGA, S ;

DAS, R ;

SOPORY, SK .

JOURNAL OF PLANT PHYSIOLOGY, 1987, 129 (1-2) :149-153

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5]

CARRINGTON SJ, 1986, IRCS MED SCI-BIOCHEM, V14, P763

[6]

Dakin HD, 1913, J BIOL CHEM, V14, P155

[7]

Dakin HD, 1913, J BIOL CHEM, V14, P423

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9]

Mannervik B., 1980, ENZYMATIC BASIS DETO, VVolume 2, P263

[10]

MERRIL CR, 1983, METHOD ENZYMOL, V96, P230

← 1 2 →