EFFECTIVE DIPLOIDIZATION OF MICROSPORE-DERIVED HAPLOIDS OF RAPE (BRASSICA-NAPUS L) BY INVITRO COLCHICINE TREATMENT

被引：24

作者：

MATHIAS, R ^{[1
]}

ROBBELEN, G ^{[1
]}

机构：

[1] UNIV GOTTINGEN, INST PFLANZENBAU & PFLANZENZUCHTUNG, VON SIEBOLD STR 8, W-3400 GOTTINGEN, GERMANY

来源：

PLANT BREEDING | 1991年 / 106卷 / 01期

关键词：

BRASSICA NAPUS; MICROSPORE CULTURE; HAPLOIDS; COLCHICINE TREATMENT; DIPLOIDIZATION;

D O I：

10.1111/j.1439-0523.1991.tb00483.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A technique for the in vitro colchicine treatment of microspore-derived haploids of rape (Brassica napus L.) is presented. This allows effective and time-saving chromosome doubling. The most effective method consists of an application of 50 mg/l colchicine in a liquid B5 medium to the rooted microspore-derived plantlets for 4-8 days. By this treatment more than 50% of the plants developed at least diploid sectors. A change of medium after some days may even increase this diploidization rate.

引用

页码：82 / 84

页数：3

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