MOLECULAR EPIDEMIOLOGY OF VIBRIO-CHOLERAE O1 ISOLATED IN NEPAL BY SOUTHERN HYBRIDIZATION WITH A CHOLERA-TOXIN GENE PROBE

被引：0
|
作者：
YAMAMOTO, K
SHRESTHA, J
IIDA, T
YOH, M
HONDA, T
机构：
来源：
JOURNAL OF DIARRHOEAL DISEASES RESEARCH | 1995年 / 13卷 / 02期
关键词：
VIBRIO CHOLERAE; EPIDEMIOLOGY; CHOLERA TOXIN; HYBRIDIZATION; OLIGONUCLEOTIDES; GENES; BACTERIAL; EPIDEMIOLOGIC METHODS;
D O I：
暂无
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
A cholera epidemic broke out in 1992 due to Vibrio cholerae O1 biotype El Tor in the eastern and southern belt of Nepal mainly among the Bhutanese refugees. Restriction fragment profiles (RFP) of DNA fragments of V. cholerae O1 isolates hybridized with an enzyme-labelled oligonucleotide probe for cholera toxin gene (ctx) by Southern Hybridization were compared. The probe hybridized with the 13- and 8-kb fragments of PstI-digested total DNA in all isolates observed in the epidemic. This RFP in the Nepalese strain was not observed in the strains isolated during other epidemics but was observed in the strains isolated from the exported marine products from Taiwan and Thailand.
引用
收藏
页码：113 / 117
页数：5
相关论文
共 50 条
  • [41] VARIATION IN EPITOPES OF THE B-SUBUNIT OF VIBRIO-CHOLERAE NON-O1 AND VIBRIO-MIMICUS CHOLERA TOXINS
    TAMPLIN, ML
    JALALI, R
    AHMED, MK
    COLWELL, RR
    CANADIAN JOURNAL OF MICROBIOLOGY, 1990, 36 (06) : 409 - 413
  • [42] Cholera outbreak caused by drug resistant Vibrio cholerae serogroup O1 biotype ElTor serotype Ogawa in Nepal; a cross-sectional study
    Gupta, Pappu Kumar
    Pant, Narayan Dutt
    Bhandari, Ramkrishna
    Shrestha, Padma
    ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL, 2016, 5
  • [43] Molecular characterization of Vibrio cholerae O1 strains circulating in Assam: a north eastern state of India
    Sharma, Ajanta
    Dutta, Bornali Sarmah
    Rabha, Debajit
    Rasul, Elmy Samsun
    Hazarika, Naba Kumar
    IRANIAN JOURNAL OF MICROBIOLOGY, 2021, 13 (05) : 583 - 591
  • [44] Molecular characterization reveals involvement of altered el tor biotype Vibrio cholerae O1 strains in cholera outbreak at Hyderabad, India
    Ajay Kumar Goel
    Meenu Jain
    Pramod Kumar
    Pennagaram Sarguna
    Meera Bai
    Neha Ghosh
    Natrajan Gopalan
    The Journal of Microbiology, 2011, 49 : 280 - 284
  • [45] Molecular characterization of ICEVchVie0 and its disappearance in Vibrio cholerae O1 strains isolated in 2003 in Vietnam
    Bani, Stefania
    Mastromarino, Patrizia Nina
    Ceccarelli, Daniela
    Le Van, An
    Salvia, Anna Maria
    Viet, Quynh Tram Ngo
    Hai, Duong Huynh
    Bacciu, Donatella
    Cappuccinelli, Piero
    Colombo, Mauro Maria
    FEMS MICROBIOLOGY LETTERS, 2007, 266 (01) : 42 - 48
  • [46] Characteristics of Vibrio cholerae O1 isolated from water of the River Ganga, Varanasi, India
    Mohaptra, S. S.
    Mantri, C. K.
    Bhotra, T.
    Singh, D. V.
    INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2015, 33 (04) : 507 - 515
  • [47] CONSTRUCTION OF PLASMIDS USEFUL FOR PRODUCTION OF THE B-SUBUNIT OF CHOLERA-TOXIN FROM VIBRIO-CHOLERAE OR A HEAT-LABILE ENTEROTOXIN FROM ENTEROTOXIGENIC ESCHERICHIA-COLI
    TSUJI, T
    KATO, M
    KATO, Y
    KAWASE, H
    IMAMURA, S
    MIYAMA, A
    EUROPEAN JOURNAL OF EPIDEMIOLOGY, 1994, 10 (04) : 393 - 398
  • [48] SURVIVAL OF CULTURABLE VIBRIO-CHOLERAE O1 AND NON O1 IN SEAWATER, FRESH-WATER AND WASTE-WATER AND EFFECT OF THE WATER ENVIRONMENT ON ENTEROTOXIN PRODUCTION
    SATO, MIZ
    SANCHEZ, PS
    RIVERA, IG
    MARTINS, MT
    REVISTA DE MICROBIOLOGIA, 1995, 26 (02): : 83 - 89
  • [49] Molecular tracking of the lineage of strains of Vibrio cholerae O1 biotype El Tor associated with a cholera outbreak in Andaman and Nicobar Islands, India
    Roy, S
    Dutta, B
    Ghosh, AR
    Sugunan, AP
    Nandy, RK
    Bhattacharya, SK
    Sehgal, SC
    TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2005, 10 (06) : 604 - 611
  • [50] Phenotypic and genetic characterization of Vibrio cholerae O1 clinical isolates collected through national antimicrobial resistance surveillance network in Nepal
    Shakya, Geeta
    Kim, Dong Wook
    Clemens, John D.
    Malla, Sarala
    Upadhyaya, Bishnu Prasad
    Dumre, Shyam Prakash
    Shrestha, Sirjana Devi
    Adhikari, Shailaja
    Sharma, Supriya
    Rijal, Nisha
    Shrestha, Sanjaya K.
    Mason, Carl
    Kansakar, Palpasa
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28 (08) : 2671 - 2678
12345