MOLECULAR EPIDEMIOLOGY OF VIBRIO-CHOLERAE O1 ISOLATED IN NEPAL BY SOUTHERN HYBRIDIZATION WITH A CHOLERA-TOXIN GENE PROBE

被引：0
|
作者：
YAMAMOTO, K
SHRESTHA, J
IIDA, T
YOH, M
HONDA, T
机构：
来源：
JOURNAL OF DIARRHOEAL DISEASES RESEARCH | 1995年 / 13卷 / 02期
关键词：
VIBRIO CHOLERAE; EPIDEMIOLOGY; CHOLERA TOXIN; HYBRIDIZATION; OLIGONUCLEOTIDES; GENES; BACTERIAL; EPIDEMIOLOGIC METHODS;
D O I：
暂无
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
A cholera epidemic broke out in 1992 due to Vibrio cholerae O1 biotype El Tor in the eastern and southern belt of Nepal mainly among the Bhutanese refugees. Restriction fragment profiles (RFP) of DNA fragments of V. cholerae O1 isolates hybridized with an enzyme-labelled oligonucleotide probe for cholera toxin gene (ctx) by Southern Hybridization were compared. The probe hybridized with the 13- and 8-kb fragments of PstI-digested total DNA in all isolates observed in the epidemic. This RFP in the Nepalese strain was not observed in the strains isolated during other epidemics but was observed in the strains isolated from the exported marine products from Taiwan and Thailand.
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页码：113 / 117
页数：5
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