GENOME FLUX IN TOMATO AUTO-TROPHIC AND AUXO-TROPHIC CELL CLONES CULTURED IN DIFFERENT AUXIN CYTOKININ EQUILIBRIA .1. DNA MULTIPLICITY AND METHYLATION LEVELS

被引：15

作者：

BOGANI, P ^{[1
]}

SIMONI, A ^{[1
]}

BETTINI, P ^{[1
]}

MUGNAI, M ^{[1
]}

PELLEGRINI, MG ^{[1
]}

BUIATTI, M ^{[1
]}

机构：

[1] UNIV FLORENCE, DEPT ANIM BIOL & GENET, FLORENCE, ITALY

来源：

GENOME | 1995年 / 38卷 / 05期

关键词：

TOMATO CLONES; SOMACLONAL VARIATION; METHYLATION; DNA AMPLIFICATION;

D O I：

10.1139/g95-119

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An analysis of the effect of changing physiological conditions on genetic stability, in terms of epigenetic changes, such as DNA, methylation patterns, and multiplicity of repetitive DNA, was carried out on tomato cell clones grown on media supplemented with different auxin/cytokinin ratios. The effect of endogenous variation in phytohormone equilibria was also indirectly analysed through a comparison of auxotrophic or habituated (autotrophic) cell clones and the differentiated leaf tissue. The data obtained showed significant variation in methylation and multiplicity levels both between clones and between treatments, clearly suggesting a contemporary influence of exogenous hormonal treatments and of the initial/endogenous physiological state of the treated tissue on both phenomena studied.

引用

页码：902 / 912

页数：11

共 39 条

[21] SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE [J].

LABARCA, C ;

PAIGEN, K .

ANALYTICAL BIOCHEMISTRY, 1980, 102 (02) :344-352

[22] DNA METHYLATION OF EMBRYOGENIC CARROT CELL-CULTURES AND ITS VARIATIONS AS CAUSED BY MUTATION, DIFFERENTIATION, HORMONES AND HYPOMETHYLATING DRUGS [J].

LOSCHIAVO, F ;

PITTO, L ;

GIULIANO, G ;

TORTI, G ;

NUTIRONCHI, V ;

MARAZZITI, D ;

VERGARA, R ;

ORSELLI, S ;

TERZI, M .

THEORETICAL AND APPLIED GENETICS, 1989, 77 (03) :325-331

[23]

Maniatis T., 1982, MOL CLONING

[24] REVERSIBLE METHYLATION AND INACTIVATION OF MARKER GENES IN SEQUENTIALLY TRANSFORMED TOBACCO PLANTS [J].

MATZKE, MA ;

PRIMIG, M ;

TRNOVSKY, J ;

MATZKE, AJM .

EMBO JOURNAL, 1989, 8 (03) :643-649

[25] HERITABLE VARIATION IN PLANT-CELL CULTURE [J].

MEINS, F .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1983, 34 :327-346

[26] HABITUATION - HERITABLE VARIATION IN THE REQUIREMENT OF CULTURED PLANT-CELLS FOR HORMONES [J].

MEINS, F .

ANNUAL REVIEW OF GENETICS, 1989, 23 :395-408

[27]

MII M, 1989, HDB CELL CULTURE, P284

[28] DNA VARIATION IN TISSUE-CULTURE-DERIVED RICE PLANTS [J].

MULLER, E ;

BROWN, PTH ;

HARTKE, S ;

LORZ, H .

THEORETICAL AND APPLIED GENETICS, 1990, 80 (05) :673-679

[29] A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].

MURASHIGE, T ;

SKOOG, F .

PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497

[30]

Nagl W., 1990, BIOTECHNOLOGY AGR FO, P153

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