GENOME FLUX IN TOMATO AUTO-TROPHIC AND AUXO-TROPHIC CELL CLONES CULTURED IN DIFFERENT AUXIN CYTOKININ EQUILIBRIA .1. DNA MULTIPLICITY AND METHYLATION LEVELS

被引：15

作者：

BOGANI, P ^{[1
]}

SIMONI, A ^{[1
]}

BETTINI, P ^{[1
]}

MUGNAI, M ^{[1
]}

PELLEGRINI, MG ^{[1
]}

BUIATTI, M ^{[1
]}

机构：

[1] UNIV FLORENCE, DEPT ANIM BIOL & GENET, FLORENCE, ITALY

来源：

GENOME | 1995年 / 38卷 / 05期

关键词：

TOMATO CLONES; SOMACLONAL VARIATION; METHYLATION; DNA AMPLIFICATION;

D O I：

10.1139/g95-119

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An analysis of the effect of changing physiological conditions on genetic stability, in terms of epigenetic changes, such as DNA, methylation patterns, and multiplicity of repetitive DNA, was carried out on tomato cell clones grown on media supplemented with different auxin/cytokinin ratios. The effect of endogenous variation in phytohormone equilibria was also indirectly analysed through a comparison of auxotrophic or habituated (autotrophic) cell clones and the differentiated leaf tissue. The data obtained showed significant variation in methylation and multiplicity levels both between clones and between treatments, clearly suggesting a contemporary influence of exogenous hormonal treatments and of the initial/endogenous physiological state of the treated tissue on both phenomena studied.

引用

页码：902 / 912

页数：11

共 39 条

[1] RAPID CHANGES IN AMPLIFICATION AND METHYLATION PATTERN OF GENOMIC DNA IN CULTURED CARROT ROOT EXPLANTS (DAUCUS-CAROTA L) [J].

ARNHOLDTSCHMITT, B .

THEORETICAL AND APPLIED GENETICS, 1993, 85 (6-7) :793-800

[2] VARIABLE METHYLATION AND DIFFERENTIAL REPLICATION OF GENOMIC DNA IN CULTURED CARROT ROOT EXPLANTS DURING GROWTH INDUCTION AS INFLUENCED BY HORMONAL TREATMENTS [J].

ARNHOLDTSCHMITT, B ;

HOLZAPFEL, B ;

SCHILLINGER, A ;

NEUMANN, KH .

THEORETICAL AND APPLIED GENETICS, 1991, 82 (03) :283-288

[3]

BASHKITE EA, 1979, BIOL NAUKI, V7, P23

[4]

BASHKITE EA, 1980, BIOL NAUKI, V4, P103

[5] QUANTITATIVE VARIATIONS OF NUCLEAR-DNA DURING PLANT DEVELOPMENT - A CRITICAL ANALYSIS [J].

BASSI, P .

BIOLOGICAL REVIEWS, 1990, 65 (03) :185-225

[6] DNA METHYLATION IN PLANTS AND ITS ROLE IN TISSUE-CULTURE [J].

BROWN, PTH .

GENOME, 1989, 31 (02) :717-729

[7]

Buiatti M., 1993, Creating genetic variation in ornamentals. Proceedings of the XVIIth Symposium of the European Association for Research on Plant Breeding, EUCARPIA, Sanremo, Italy, 1-5 March 1993., P5

[8]

BUIATTI M, 1977, APPLIED FUNDAMENTAL, P358

[9]

CHVOYKA L, 1978, Biokhimiya, V43, P996

[10] ENVIRONMENTALLY INDUCED DNA CHANGES IN PLANTS [J].

CULLIS, CA .

CRC CRITICAL REVIEWS IN PLANT SCIENCES, 1983, 1 (02) :117-131

← 1 2 3 4 →