INTERLEUKIN-1-BETA ANTAGONIZES PHENOBARBITAL INDUCTION OF SEVERAL MAJOR CYTOCHROMES P450 IN ADULT-RAT HEPATOCYTES IN PRIMARY CULTURE

被引：46
作者：
ABDELRAZZAK, Z [1 ]
CORCOS, L [1 ]
FAUTREL, A [1 ]
GUILLOUZO, A [1 ]
机构：
[1] HOP PONTCHAILLOU,INSERM,U49,F-35033 RENNES,FRANCE
来源：
FEBS LETTERS | 1995年 / 366卷 / 2-3期
关键词：
ADULT RAT HEPATOCYTE; CYTOCHROME P450; PHENOBARBITAL; CYTOKINE; INTERLEUKIN-1-BETA; INTERLEUKIN-6;
D O I：
10.1016/0014-5793(95)00513-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have investigated the effects of interleukin (IL)-1 beta and IL6 on expression and phenobarbital (PB) induction of ethoxyresorufin O-deethylase (EROD) and pentoxyresorufin O-deethylase (PROD) activities, as well as on mRNA levels of cytochromes P450 (CYP) 1A, 2B, 2C, 2E and 3A, in rat hepatocytes in primary culture. IL6 slightly antagonized PB-induced PROD activity. Strikingly, IL1 beta strongly inhibited basal EROD and PROD activities, and fully blocked their induction by PB in a dose-dependent fashion. Furthermore IL1 beta completely suppressed PB induction of all CYP mRNAs analyzed. Our results demonstrate that IL1 beta can suppress basal CYP activities, as well as PB-inducible expression of five CYP mRNAs in rat hepatocytes in primary culture.
引用
收藏
页码：159 / 164
页数：6
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