INTERLEUKIN-1-BETA ANTAGONIZES PHENOBARBITAL INDUCTION OF SEVERAL MAJOR CYTOCHROMES P450 IN ADULT-RAT HEPATOCYTES IN PRIMARY CULTURE

被引：46

作者：

ABDELRAZZAK, Z ^{[1
]}

CORCOS, L ^{[1
]}

FAUTREL, A ^{[1
]}

GUILLOUZO, A ^{[1
]}

机构：

[1] HOP PONTCHAILLOU,INSERM,U49,F-35033 RENNES,FRANCE

来源：

FEBS LETTERS | 1995年 / 366卷 / 2-3期

关键词：

ADULT RAT HEPATOCYTE; CYTOCHROME P450; PHENOBARBITAL; CYTOKINE; INTERLEUKIN-1-BETA; INTERLEUKIN-6;

D O I：

10.1016/0014-5793(95)00513-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the effects of interleukin (IL)-1 beta and IL6 on expression and phenobarbital (PB) induction of ethoxyresorufin O-deethylase (EROD) and pentoxyresorufin O-deethylase (PROD) activities, as well as on mRNA levels of cytochromes P450 (CYP) 1A, 2B, 2C, 2E and 3A, in rat hepatocytes in primary culture. IL6 slightly antagonized PB-induced PROD activity. Strikingly, IL1 beta strongly inhibited basal EROD and PROD activities, and fully blocked their induction by PB in a dose-dependent fashion. Furthermore IL1 beta completely suppressed PB induction of all CYP mRNAs analyzed. Our results demonstrate that IL1 beta can suppress basal CYP activities, as well as PB-inducible expression of five CYP mRNAs in rat hepatocytes in primary culture.

引用

页码：159 / 164

页数：6

共 48 条

[1]

ABDELRAZZAK Z, 1994, MOL PHARMACOL, V46, P1100

[2]

ABDELRAZZAK Z, 1993, MOL PHARMACOL, V44, P707

[3]

ADESNIK M, 1981, J BIOL CHEM, V256, P340

[4] CDNA CLONES FOR LIVER CYTOCHROME-P-450S FROM INDIVIDUAL AROCLOR-TREATED RATS - CONSTITUTIVE EXPRESSION OF A NEW P-450 GENE RELATED TO PHENOBARBITAL-INDUCIBLE FORMS [J].