RECOGNITION OF CHIMERIC SMALL-SUBUNIT RIBOSOMAL DNAS COMPOSED OF GENES FROM UNCULTIVATED MICROORGANISMS

被引:149
作者
KOPCZYNSKI, ED [1 ]
BATESON, MM [1 ]
WARD, DM [1 ]
机构
[1] MONTANA STATE UNIV,DEPT MICROBIOL,BOZEMAN,MT 59717
关键词
D O I
10.1128/AEM.60.2.746-748.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
When PCR nas used to recover small-subunit (SSU) rRNA genes from a hot spring cyanobacterial mat community, chimeric SSU rRNA sequences which exhibited little or no secondary structural abnormality were recovered. They were revealed as chimeras of SSU rRNA genes of uncultivated species through separate phylogenetic analysis of short sequence domains.
引用
收藏
页码:746 / 748
页数:3
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