CRYSTALLIZATION OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C FROM BACILLUS-CEREUS

被引：8

作者：

BULLOCK, TL ^{[1
]}

RYAN, M ^{[1
]}

KIM, SL ^{[1
]}

REMINGTON, SJ ^{[1
]}

GRIFFITH, OH ^{[1
]}

机构：

[1] UNIV OREGON, INST MOLEC BIOL, EUGENE, OR 97403 USA

来源：

BIOPHYSICAL JOURNAL | 1993年 / 64卷 / 03期

关键词：

D O I：

10.1016/S0006-3495(93)81439-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Phosphatidylinositol-specific phospholipase C (PI-PLC) cleaves phosphoinositides into two parts, lipid-soluble diacylglycerol and the water-soluble phosphorylated inositol. Two crystal forms of Bacillus cereus PI-PLC have been obtained by the vapor diffusion technique. Hexagonal crystals were grown from solutions containing polyethylene glycol (PEG; 4,000 to 8,000 D). The space group of these hexagonal crystals is P6(1)22 (or the enantiomorphic space group P6(5)22), with cell constants a = b = 133 angstrom, and c = 231 angstrom. The crystals diffract to 2.8 angstrom. The second crystalline form was grown from a two-phase PEG (600 D)-sodium citrate solution. The phase diagram and PI-PLC distribution between phases has been determined. The enzyme crystallizes from the PEG-rich phase. The crystals are orthorhombic with space group P2(1)2(1)2(1) (a = 45 angstrom, b = 46 angstrom, c = 160 angstrom), and contain one PI-PLC monomer per asymmetric unit. The orthorhombic crystals diffract to 2.5 angstrom. Both the hexagonal and orthorhombic forms are suitable for crystallographic studies.

引用

页码：784 / 791

页数：8

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