NORADRENERGIC INHIBITION AND VOLTAGE-DEPENDENT FACILITATION OF OMEGA-CONOTOXIN-SENSITIVE CA CHANNELS IN INSULIN-SECRETING RINM5F CELLS

被引：27

作者：

AICARDI, G

POLLO, A

SHER, E

CARBONE, E

机构：

[1] DIPARTIMENTO ANAT & FISIOL UMANA,CORSO RAFFAELLO 30,I-10125 TURIN,ITALY

[2] INST HUMAN PHYSIOL,BOLOGNA,ITALY

[3] CNR,CTR CYTOPHARMACOL,DEPT PHARMACOL,I-20133 MILAN,ITALY

来源：

FEBS LETTERS | 1991年 / 281卷 / 1-2期

关键词：

INSULIN-SECRETING CELL; CA CHANNEL; GTP-ANALOG; NORADRENALINE; OMEGA-CONOTOXIN;

D O I：

10.1016/0014-5793(91)80393-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We found that, besides dihydropyridine-sensitive Ca channels, insulin-secreting RINm5F cells also contain a minority (15-25%) of omega-conotoxin (omega-CgTx)-sensitive channels that show a high-affinity binding to [I-125]omega-CgTx (K(d) 51 pM). Noradrenaline (NA, 10-mu-M) slows down Ca-channel activation in these cells and produces a sizeable reduction of Ca currents that is relieved by strong pre-conditioning depolarizations (facilitation). The action of NA is mimicked by intracellular application of GTP-gamma-S and is prevented by pertussis toxin (PTX) or by cell pre-incubation with omega-CgTx. This suggests specific noradrenergic inhibition of omega-CgTx-sensitive Ca channels that is modulated by membrane potentials and PTX-sensitive G-protein activation.

引用

页码：201 / 204

页数：4

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