EFFECTS OF HUMAN SERA AND HUMAN SERUM-ALBUMIN ON MOUSE EMBRYO CULTURE

被引：13

作者：

LEVEILLE, MC ^{[1
]}

CARNEGIE, J ^{[1
]}

TANPHAICHITR, N ^{[1
]}

机构：

[1] UNIV OTTAWA,OTTAWA CIV HOSP,LOEB RES INST,DEPT OBSTET & GYNECOL,OTTAWA K1Y 4E9,ONTARIO,CANADA

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 01期

关键词：

MOUSE EMBRYO CULTURE; HUMAN SERA; HUMAN SERUM ALBUMIN; BOVINE SERUM ALBUMIN;

D O I：

10.1007/BF01204114

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Human proteins normally used to supplement human in vitro fertilization-embryo transfer (IVF-ET) culture media were tested for their effects on mouse embryo development from the zygote stage. These proteins included follicular and luteal-phase maternal sera, fetal cord sera, and both human and bovine serum albumin. Our results revealed that both maternal and fetal cord sera did not permit mouse blastocyst formation. Furthermore, predialysis of the human maternal sera and removal of IgG by protein A column chromatography did not improve their support of mouse embryonic development to the blastocyst stage. Similar detrimental effects were observed with maternal sera from term-pregnant IVF-ET patients. Interestingly, these serum samples had supported the in vitro growth of the human zygotes which resulted in these patients' pregnancies. Only some batches of human serum albumin supported mouse blastocyst formation, whereas all sources of bovine serum albumin were effective in this regard. These results raise the question of the suitability of the mouse embryo culture system as a quality control for the testing of protein supplements for human IVF-ET.

引用

页码：45 / 52

页数：8

共 25 条

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