IDENTIFICATION OF A SMALL NA+/H+ EXCHANGER-LIKE MESSAGE IN THE RABBIT MYOCARDIUM

被引：14

作者：

DYCK, JRB

LOPASCHUK, GD

FLIEGEL, L

机构：

[1] UNIV ALBERTA,FAC MED,DEPT PEDIAT,CARDIOVASC DIS RES GRP,EDMONTON T6G 2E1,ALBERTA,CANADA

[2] UNIV ALBERTA,FAC MED,DEPT BIOCHEM,CARDIOVASC DIS RES GRP,EDMONTON T6G 2E1,ALBERTA,CANADA

[3] UNIV ALBERTA,FAC MED,DEPT PHARMACOL,CARDIOVASC DIS RES GRP,EDMONTON T6G 2E1,ALBERTA,CANADA

来源：

FEBS LETTERS | 1992年 / 310卷 / 03期

关键词：

NA+/H+ EXCHANGER; MYOCARDIUM; MESSENGER RNA; ACIDOSIS; ISCHEMIA;

D O I：

10.1016/0014-5793(92)81343-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined the Na+/H+ exchanger message in isolated perfused rabbit hearts using Northern blot analysis with cDNA encoding for the rabbit cardiac Na+/H+ exchanger. A cDNA probe from the coding region of the rabbit myocardial Na+/H+ exchanger hybridized to mRNA of 5 kb under high stringency, and to a second 3.8 kb mRNA species under low stringency. When Northern blots were re-probed with a section of the 3'-untranslated region of the cDNA, the 5 kb message was apparent while the smaller 3.8 kb message was not. If isolated working rabbit hearts were subjected to ischemia we observed increases in the 3.8 kb message. Overall, the results show that a 3.8 kb mRNA product, which is homologous to the amiloride sensitive Na+/H+ exchanger, exists in the myocardium and increases during ischemia in the myocardium.

引用

页码：255 / 259

页数：5

共 24 条

[1] NA+-H+ EXCHANGER IN PROXIMAL CELLS ISOLATED FROM KIDNEY .2. SHORT-TERM REGULATION BY GLUCOCORTICOIDS [J].