WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD

被引：4213

作者：

FLEISCHMANN, RD

ADAMS, MD

WHITE, O

CLAYTON, RA

KIRKNESS, EF

KERLAVAGE, AR

BULT, CJ

TOMB, JF

DOUGHERTY, BA

MERRICK, JM

MCKENNEY, K

SUTTON, G

FITZHUGH, W

FIELDS, C

GOCAYNE, JD

SCOTT, J

SHIRLEY, R

LIU, LI

GLODEK, A

KELLEY, JM

WEIDMAN, JF

PHILLIPS, CA

SPRIGGS, T

HEDBLOM, E

COTTON, MD

UTTERBACK, TR

HANNA, MC

NGUYEN, DT

SAUDEK, DM

BRANDON, RC

FINE, LD

FRITCHMAN, JL

FUHRMANN, JL

GEOGHAGEN, NSM

GNEHM, CL

MCDONALD, LA

SMALL, KV

FRASER, CM

SMITH, HO

VENTER, JC

机构：

[1] INST GENOM RES, GAITHERSBURG, MD 20878 USA

[2] JOHNS HOPKINS UNIV, SCH MED, BALTIMORE, MD 21205 USA

[3] SUNY BUFFALO, DEPT MICROBIOL, BUFFALO, NY 14214 USA

[4] NIST, GAITHERSBURG, MD 20878 USA

来源：

SCIENCE | 1995年 / 269卷 / 5223期

关键词：

D O I：

10.1126/science.7542800

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

引用

页码：496 / 512

页数：17

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