IMMUNOCYTOCHEMICAL DEMONSTRATION OF WHEAT-GERM-AGGLUTININ (WGA)-RECEPTORS ON THE CELL-SURFACE OF GUINEA-PIG PERITONEAL-MACROPHAGES

被引：1

作者：

KRIEGSMANN, J

NESER, F

BRAUER, R

机构：

[1] Institute of Pathology, Friedrich Schiller University Jena

来源：

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY | 1993年 / 45卷 / 2-3期

关键词：

WHEAT GERM AGGLUTININ (WGA) RECEPTORS; RECEPTORS; WHEAT GERM AGGLUTININ; MACROPHAGES; PERITONEAL; IMMUNOGOLD TECHNIQUE; CELL SURFACE; BINDING SITES; WGA-ANTI WGA-PROTEIN A-GOLD TECHNIQUE; WGA/BSA (BOVINE SERUM ALBUMIN)-GOLD TECHNIQUE; WGA (WHEAT GERM AGGLUTININ); PERITONEAL MACROPHAGES;

D O I：

10.1016/S0940-2993(11)80471-9

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

WGA-receptors on the surface of resident guinea pig peritoneal macrophages were investigated. Macrophages were characterized by the endogenous peroxidase pattern. Two different immunogold techniques, the indirect WGA-anti WGA-protein A-gold technique and the direct WGA/BSA-gold technique were compared to evaluate their suitability for quantification of WGA binding sites on the cell surface. Because of the higher binding specificity the direct technique should be preferred for quantitative studies.

引用

页码：93 / 97

页数：5