MUTATIONAL ANALYSIS OF THE HELPER COMPONENT-PROTEINASE GENE OF A POTYVIRUS - EFFECTS OF AMINO-ACID SUBSTITUTIONS, DELETIONS, AND GENE REPLACEMENT ON VIRULENCE AND APHID TRANSMISSIBILITY

被引：163

作者：

ATREYA, CD ^{[1
]}

PIRONE, TP ^{[1
]}

机构：

[1] UNIV KENTUCKY, DEPT PLANT PATHOL, LEXINGTON, KY 40506 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 24期

关键词：

CHIMERIC PLANT VIRUS; INSECT TRANSMISSION;

D O I：

10.1073/pnas.90.24.11919

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have previously provided evidence that amino acid substitutions within the N-terminal portion of the helper component-proteinase (HC-Pro) from tobacco vein mottling virus (TVMV), in particular at Lys-307, not only affect the aphid transmission activity of HC-Pro but also have a significant effect on TVMV virulence. In the present study amino acids which differ in their charge properties were substituted at position 307. A highly basic residue was required to retain helper component activity and virulence. Deletion and insertion mutagenesis in the 5' terminus of the HC-Pro gene suggested that this RNA domain may be an essential element for TVMV infectivity. Replacement of the TVMV HC-Pro gene with that from another potyvirus, zucchini yellow mosaic virus, maintained infectivity and aphid transmissibility of the chimeric virus, although symptoms were attenuated. Our results suggest that, in addition to its importance in aphid transmission, the HC-Pro gene may be of general importance in regulating virulence of potyviruses, possibly by interaction of these sequences with the host.

引用

页码：11919 / 11923

页数：5

共 22 条

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