MOLECULAR-CLONING OF DNA FROM SPECIFIC CHROMOSOMAL REGIONS BY MICRODISSECTION AND SEQUENCE-INDEPENDENT AMPLIFICATION OF DNA

被引：66
作者：
JOHNSON, DH
机构：
[1] Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Miami, FL 33136
来源：
GENOMICS | 1990年 / 6卷 / 02期
关键词：
D O I：
10.1016/0888-7543(90)90563-A
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method that permits the in vitro amplification and cloning of DNA dissected from specific regions of a chromosome and does not require prior knowledge of the DNA sequence is described. DNA from several different chromosomal loci in the Drosophila melanogaster genome has been isolated by this method. Although the procedure was developed to permit the isolation of DNA sequences from serial sections of a single microdissected polytene chromosome, it should be useful for obtaining DNA clones from specific regions of the nonpolytene chromosomes of other organisms as well. © 1990.
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页码：243 / 251
页数：9
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