A NEW FLUORESCENCE ENHANCEMENT SOLUTION FOR EUROPIUM-BASED TIME-RESOLVED FLUOROIMMUNOASSAYS

被引：10

作者：

DEGAN, P

ABBONDANDOLO, A

MONTAGNOLI, G

机构：

[1] CNR,IST BIOFIS,I-56100 PISA,ITALY

[2] UNIV PISA,DIPARTIMENTO SCI ANAT FISIOL & PROD ANIM,I-56100 PISA,ITALY

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1990年 / 5卷 / 03期

关键词：

D O I：

10.1002/bio.1170050312

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cationic detergent is proposed as suitable insulating agent for the preparation of a fluorescence enhancement solution useful for europium-based time-resolved fluoroimmunoassays. Luminescence from europium ions at concentration as low as 0.5 pmol/l can be detected in solution containing 1.6 mmol/l thenoyltrifluoroacetone, 110.5 mumol/l Adogen 464 and 0.1% Tween 20, in the presence of 0.5 mol/l NaCl. A competitive TR-FIA for rabbit IgG is described, showing that the new enhancement solution allows a sensitivity comparable to that of the high performance LKB Wallac DELFIA.

引用

页码：207 / 212

页数：6

共 16 条

[1]

DAHLEN P, 1987, ANAL BIOCHEM, V164, P463

[2] SEPARATION OF ESCHERICHIA-COLI RIBOSOMAL RIBONUCLEIC ACIDS BY REVERSED-PHASE CHROMATOGRAPHY [J].