CHARACTERIZATION OF RECEPTORS INVOLVED IN DOPAMINE-INDUCED ACTIVATION OF PHOSPHOLIPASE-C IN RAT RENAL-CORTEX

Dopamine (DA) is reported to stimulate phospholipase-C (PL-C) in rat renal cortex. Inasmuch as DA activates alpha adrenoceptors and DA receptors, the relative contribution of these receptors to DA-induced activation of PL-C is not yet established. We examined the effect of DA on PL-C activity in rat renal cortical slices prelabeled with myo-2-[H-3]inositol in the presence of Li+. PL-C activity was expressed as fractional release (FR) of combined [H-3]inositol phosphates expressed as dpm inositol phosphates accumulated/total dpm incorporated x 100. DA (1 mM) produced time-dependent increases in FR up to 60 min. DA (1, 3 and 10 mM) produced 61%, 88% and 110% increases in FR over control. When DA was given in the presence of SCH 23390, a selective DA-1 receptor antagonist, the increase in FR was significantly reduced to 33%, 51% and 62%, respectively, but the increase in FR remained unaffected in the presence of a DA-2 receptor antagonist, domperidone (30-mu-M). Phentolamine (10-mu-M) also inhibited the response to DA to 41%, 47% and 43% at the respective concentrations. DA-induced stimulation of PL-C was completely abolished in the combined presence of both SCH 23390 (30-mu-M) and phentolamine (10-mu-M). SCH 23390, domperidone or phentolamine alone did not significantly change the basal PL-C activity in renal cortical slices. These results demonstrate that 1) DA stimulates PL-C in rat renal cortex via activation receptors are not involved in this response; and 2) during normal sodium intake, intrarenal DA does not modulate the PL-C activity in rat renal cortex.