COOPERATIVE BINDING OF THE RED-CLOVER NECROTIC MOSAIC-VIRUS MOVEMENT PROTEIN TO SINGLE-STRANDED NUCLEIC-ACIDS

被引:87
作者
OSMAN, TAM [1 ]
HAYES, RJ [1 ]
BUCK, KW [1 ]
机构
[1] IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOL,LONDON SW7 2BB,ENGLAND
关键词
D O I
10.1099/0022-1317-73-2-223
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The movement protein of red clover necrotic mosaic dianthovirus was produced in Escherichia coli using an expression vector. Gel retardation analysis and u.v. cross-linking studies showed that the movement protein bound cooperatively to ssRNA and ssDNA, but not to dsDNA. Binding competition experiments established that the movement protein bound to ssRNA and ssDNA with similar affinities and that the binding was not sequence-specific in the experimental conditions employed. A truncated movement protein lacking the C-terminal 88 amino acids was also shown to bind to ssRNA.
引用
收藏
页码:223 / 227
页数:5
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