EFFECT OF OXYGEN-TENSION AND LACTATE CONCENTRATION ON KERATAN SULFATE AND CHONDROITIN SULFATE BIOSYNTHESIS IN BOVINE CORNEA

被引：13

作者：

BALDUINI, C ^{[1
]}

DELUCA, G ^{[1
]}

PASSI, A ^{[1
]}

RINDI, S ^{[1
]}

SALVINI, R ^{[1
]}

SCOTT, JE ^{[1
]}

机构：

[1] UNIV MANCHESTER,DEPT CHEM MORPHOL,MANCHESTER M13 9PL,LANCS,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1115卷 / 03期

关键词：

OXYGEN TENSION; LACTATE; CHONDROITIN SULFATE; KERATAN SULFATE; CORNEA;

D O I：

10.1016/0304-4165(92)90052-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Calf cornea slices were incubated with [U-C-14]glucose, in varying pO2 or lactate concentrations. Acid glycosaminoglycans were separated by ion-exchange chromatography after papain digestion. The percentage radioactivity incorporated into keratan sulphate increased markedly with decreased oxygen tension, whereas a concomitant relative decrease of the biosynthesis of glycosaminoglycuronans occurred. Similar results were obtained with increased lactate concentration. Our findings support the idea that keratan sulphate is a functional substitute for chondroitin sulphate in conditions of oxygen lack (Scott, J.E. and Haigh, M. (1988) J. Anat. 158, 95-108).

引用

页码：187 / 191

页数：5

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