SEROLOGICAL TECHNIQUES FOR DETECTION OF CITRUS TRISTEZA VIRUS

被引:28
作者
ROCHAPENA, MA [1 ]
LEE, RF [1 ]
机构
[1] UNIV FLORIDA,CTR AGR RES & EDUC,CTR CITRUS RES & EDUC,LAKE ALFRED,FL 33850
关键词
SDS-IMMUNODIFFUSION; SSEM; ISIF; GOLD-LABELING; ELISA; WESTERN BLOT; DOTIMMUNOBINDING; STRAIN DIFFERENTIATION;
D O I
10.1016/0166-0934(91)90109-D
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Citrus tristeza virus (CTV) is the most economically important virus disease of citrus. In the last ten years, remarkable progress has been achieved in the development and improvement of new serological methods for CTV detection so that serology has become a dependable tool for many research, extension and regulatory purposes worldwide. CTV-specific polyclonal antisera and monoclonal antibodies have been developed in different research laboratories and used extensively in a wide range of different studies. This review describes the diverse serological methods developed for CTV detection and analyzes the advantages, disadvantages, relative sensitivity, applications, and present status of each method.
引用
收藏
页码:311 / 331
页数:21
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