IMMOBILIZATION OF ASPERGILLUS-NIGER MYCELIUM ON SEEDS FOR GLUCOAMYLASE PRODUCTION

Mycelium of the glucoamylolytic mutant Aspergillus niger C-58-III was immobilized on wheat, rye, barley, pea, buckwheat and mustard seeds in repeated-batch flasks. After every 24h it was possible to obtain culture broth rich in glucoamylase. The highest yield of enzyme (66.4 U x ml(-1)) was obtained on the mustard carrier. Immobilized cells were successfully reused with high level of enzyme formation being mantained for longer period (192h). Some of the variables influencing the enzymatic activity have been standardized. Enzyme productivity reached in immobilized cells of A. niger was 1.6 times higher in comparison with free cells.