Characterizing the impact of Three Gorges Dam on the Changjiang (Yangtze River): A story of nitrogen biogeochemical cycling through the lens of nitrogen stable isotopes

The alterations of nitrogen sources and cycling within the Three Gorges Reservoir (TGR) and downstream the Changjiang were investigated to understand the impacts of the construction of the Three Gorges Dam (TGD) and anthropogenic inputs from the associated watershed. Water samples collected in October 2016 were analyzed for hydrologic parameters, nutrient concentrations, and stable isotopes of nitrate (NO3-), ammonium (NH4+) and particulate matter. Nitrate dual stable isotope values ranged from +5.8 parts per thousand to +7.1 parts per thousand and -1.9 parts per thousand to +0.4 parts per thousand for delta N-15 and delta O-18, respectively. delta N-15 values in particulate nitrogen (PN) ranged from +0.5 parts per thousand to +8.5 parts per thousand, with slightly lower values before the dam. delta N-15-NH4+ values ranged between +10.5 parts per thousand and +19.4 parts per thousand, likely reflecting the presence of ammonium assimilation throughout the TGR. The contribution of different nitrogen sources was calculated using a Bayesian mixing model. These sources, including soil organic nitrogen, ammonium fertilizer, and sewage effluent, contributed to elevated DIN concentrations within the TGR (83.2 mu M-178.5 mu M). The construction of the dam has also likely induced changes in the river environment such as ammonium assimilation in the surface waters and nitrification and/or remineralization within the deep waters of the TGR. Overall, during this investigation period, the TGR acted as a sink of PN (retaining 29%), yet negligibly influenced levels of TDN with similar to 96.5% of TDN exported to the downstream Changjiang and estuary. It is important to understand the long-term impacts of the TGD on the ecological environment of the Changjiang. This study highlights the influence that anthropogenic nitrogen sources have on the natural biogeochemical cycling within the TGR, showing the urgent need to reduce anthropogenic nitrogen pollution.