QUANTITATION OF ONCOGENE PRODUCTS BY COMPUTER-ASSISTED IMAGE-ANALYSIS AND FLOW-CYTOMETRY

被引：34

作者：

CZERNIAK, B ^{[1
]}

HERZ, F ^{[1
]}

WERSTO, RP ^{[1
]}

ALSTER, P ^{[1
]}

PUSZKIN, E ^{[1
]}

SCHWARZ, E ^{[1
]}

KOSS, LG ^{[1
]}

机构：

[1] MONTEFIORE MED CTR,ALBERT EINSTEIN COLL MED,DEPT PATHOL,111 E 210TH ST,BRONX,NY 10467

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1990年 / 38卷 / 04期

关键词：

Flow cytometry; Image analysis; Oncogene products;

D O I：

10.1177/38.4.1969431

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The use of antibodies permits the study of oncogene product expression in cells and tissues. However, quantitation of the levels of expression in immunohistochemical preparations is beset by difficulties, and the available scoring systems provide semiquantitative data at best. Here we describe the use of computer-assisted image analysis for determination of oncoprotein levels in a model system and compare the results with those generated by flow cytometric analysis. The oncogene products measured are located in the nucleus (c-myc p62 and c-fos p55), the inner surface of the membrane (c-ras p21), and both sides of the membrane (c-erbB-2 p185). In each instance, both analytic modalities yielded concordant results. Our data indicate that computer-assisted image analysis is a useful tool for quantitating cell components in immunohistochemical preparations.

引用

页码：463 / 466

页数：4

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