HIS273 OF 3-ISOPROPYLMALATE DEHYDROGENASE FROM THERMUS-THERMOPHILUS HB8 IS INVOLVED IN THE COENZYME BINDING

被引：2
|
作者：
YAOI, T
MIYAZAKI, K
OSHIMA, T
机构：
[1] Department of Life Science, Tokyo Institute of Technology, Nagatsuta
[2] National Institute of Bioscience and Human-Technology, Tsukuba 305
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 03期
关键词：
D O I：
10.1006/bbrc.1995.1720
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The coenzyme binding site of NAD(+)-dependent 3-isopropylmalate dehydrogenase from Thermus thermophilus was analyzed by chemical modification and site-directed mutagenesis, and His273 of the enzyme was identified to be involved in the coenzyme binding. (C) 1995 Academic Press, Inc.
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页码：733 / 737
页数：5
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