STRUCTURE AND VARIABILITY OF RECENTLY INSERTED ALU FAMILY MEMBERS

被引:177
作者
BATZER, MA [1 ]
KILROY, GE [1 ]
RICHARD, PE [1 ]
SHAIKH, TH [1 ]
DESSELLE, TD [1 ]
HOPPENS, CL [1 ]
DEININGER, PL [1 ]
机构
[1] ALTON OCHSNER MED FDN & OCHSNER CLIN,MOLEC GENET LAB,NEW ORLEANS,LA 70121
关键词
D O I
10.1093/nar/18.23.6793
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The HS subfamily of Alu sequences is comprised of a group of nearly identical members. Individual subfamily members share 97.7% nucleotide identity with each other and 98.9% nucleotide identity with the HS consensus sequence. Individual subfamily members are on the average 2.8 million years old, and were probably derived from a single source 'master' gene sometime after the human/great ape divergence. The recent Alu family member insertions provide a better image of the structure of Alu retroposons before they have had the opportunity to change significantly. All of the HS subfamily members are flanked by perfect direct repeats as a result of insertion at staggered nicks. The 'master' gene from which the HS subfamily members were derived had an oligo-dA rich tail at least 40 bases long. The 'master' gene is very rich in CpG dinucleotides, but nucleotide substitutions within subfamily members accumulated in a random manner typical for Alu sequences with CpG substitutions occurring 9.2 fold faster than non-CpG substitutions.
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页码:6793 / 6798
页数:6
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