ISOLATION AND CHARACTERIZATION OF A YEAST GENE, MPD1, THE OVEREXPRESSION OF WHICH SUPPRESSES INVIABILITY CAUSED BY PROTEIN DISULFIDE-ISOMERASE DEPLETION

被引：50

作者：

TACHIKAWA, H ^{[1
]}

TAKEUCHI, Y ^{[1
]}

FUNAHASHI, W ^{[1
]}

MIURA, T ^{[1
]}

GAO, XD ^{[1
]}

FUJIMOTO, D ^{[1
]}

MIZUNAGA, T ^{[1
]}

ONODERA, K ^{[1
]}

机构：

[1] UNIV TOKYO, DIV AGR & AGR LIFE SCI, BUNKYO KU, TOKYO 113, JAPAN

来源：

FEBS LETTERS | 1995年 / 369卷 / 2-3期

关键词：

PROTEIN DISULFIDE ISOMERASE; MULTICOPY SUPPRESSOR; SACCHAROMYCES CEREVISIAE;

D O I：

10.1016/0014-5793(95)00750-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MPD1, a yeast gene the overexpression of which suppresses the inviability caused by the loss of protein disulfide isomerase (PDI) was isolated and characterized. The MPD1 gene product retained a single disulfide isomerase active site sequence (APWCGHCK), an N-terminal putative signal sequence, and a C-terminal endoplasmic reticulum (ER) retention signal, and was a novel member of the PDI family, The gene product, identified in yeast extract, contained core size carbohydrates. MPD1 was not essential for growth, but overexpression of the gene suppressed the maturation defect of carboxypeptidase Y caused by PDI1 deletion, indicative of the related function to PDI in the yeast ER.

引用

页码：212 / 216

页数：5

共 37 条

[1] THE BONDS THAT TIE - CATALYZED DISULFIDE BOND FORMATION [J].