VOLTAGE-DEPENDENT CA2+ CHANNELS IN CHINESE-HAMSTER OVARY (CHO) CELLS

被引：30
作者：
SKRYMA, R [1 ]
PREVARSKAYA, N [1 ]
VACHER, P [1 ]
DUFY, B [1 ]
机构：
[1] UNIV BORDEAUX 2,NEUROPHYSIOL LAB,CNRS,URA 1200,F-33076 BORDEAUX,FRANCE
来源：
FEBS LETTERS | 1994年 / 349卷 / 02期
关键词：
CHO-CELL; CALCIUM CURRENT; CALCIUM CHANNEL;
D O I：
10.1016/0014-5793(94)00690-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Voltage-dependent Ca2+ channels were identified in CHO-K1 cells, currently used in molecular biology studies. Experimental data obtained at both macroscopic and single-channel levels using the patch-clamp technique show that the Ca2+ current in CHO cells is similar to the high-threshold L-type of Ca2+ current previously observed in excitable cells. It can be carried by Ca2+ or Ba2+ ions, blocked by both inorganic (Co2+) and organic (nifedipine, isradipine) Ca2+ channel blockers. The unitary Ca2+ channel activity was characterized by a conductance of 19 pS in 60 mM Ca2+, by single exponential distribution of open times (t = 0.84 ms) and biexponential distribution of closed times (t(f) = 1.67 ms, t(s) = 7.9 ms). However, the functional role of these Ca2+ channels in CHO cells remains unclear.
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收藏
页码：289 / 294
页数：6
相关论文
共 27 条
[1]   STABLE AND FUNCTIONAL EXPRESSION OF THE CALCIUM-CHANNEL ALPHA-1 SUBUNIT FROM SMOOTH-MUSCLE IN SOMATIC-CELL LINES [J].
BOSSE, E ;
BOTTLENDER, R ;
KLEPPISCH, T ;
HESCHELER, J ;
WELLING, A ;
HOFMANN, F ;
FLOCKERZI, V .
EMBO JOURNAL, 1992, 11 (06) :2033-2038
[2]   INACTIVATION OF CA CHANNELS [J].
ECKERT, R ;
CHAD, JE .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1984, 44 (03) :215-267
[3]   2 TYPES OF CALCIUM CHANNELS IN THE SOMATIC MEMBRANE OF NEWBORN RAT DORSAL-ROOT GANGLION NEURONS [J].
FEDULOVA, SA ;
KOSTYUK, PG ;
VESELOVSKY, NS .
JOURNAL OF PHYSIOLOGY-LONDON, 1985, 359 (FEB) :431-446
[4]   KINETIC AND PHARMACOLOGICAL PROPERTIES DISTINGUISHING 3 TYPES OF CALCIUM CURRENTS IN CHICK SENSORY NEURONS [J].
FOX, AP ;
NOWYCKY, MC ;
TSIEN, RW .
JOURNAL OF PHYSIOLOGY-LONDON, 1987, 394 :149-172
[5]   SMALL LINEAR CHLORIDE CHANNELS ARE ENDOGENOUS TO NONEPITHELIAL CELLS [J].
GABRIEL, SE ;
PRICE, EM ;
BOUCHER, RC ;
STUTTS, MJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (03) :C708-C713
[6]   DIFFERENT MODES OF CA-CHANNEL GATING BEHAVIOR FAVORED BY DIHYDROPYRIDINE CA-AGONISTS AND ANTAGONISTS [J].
HESS, P ;
LANSMAN, JB ;
TSIEN, RW .
NATURE, 1984, 311 (5986) :538-544
[7]   MUSCARINIC RECEPTOR SUBTYPES - MODULATION OF ION CHANNELS [J].
JONES, SVP .
LIFE SCIENCES, 1993, 52 (5-6) :457-464
[8]   3 TYPES OF CALCIUM CHANNELS IN THE MEMBRANE OF MOUSE SENSORY NEURONS [J].
KOSTYUK, PG ;
SHUBA, YM ;
SAVCHENKO, AN .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1988, 411 (06) :661-669
[9]  
KOSTYUK PG, 1988, CALCIUM CHANNEL STRU, P442
[10]   CHARACTERIZATION OF ENDOGENOUS SODIUM-CHANNEL GENE EXPRESSED IN CHINESE-HAMSTER OVARY CELLS [J].
LALIK, PH ;
KRAFTE, DS ;
VOLBERG, WA ;
CICCARELLI, RB .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (04) :C803-C809
123