MOLECULAR AND GENETIC-CHARACTERIZATION OF CYTOCHROME OXIDASE-NEGATIVE AEROMONAS-SALMONICIDA ISOLATED FROM COHO SALMON (ONCORHYNCHUS-KISUTCH)

被引：3

作者：

TESKA, JD

CIPRIANO, RC

SCHILL, WB

机构：

[1] U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Kearneysville, 25430, Virginia

来源：

JOURNAL OF WILDLIFE DISEASES | 1992年 / 28卷 / 04期

关键词：

AEROMONAS-SALMONICIDA; CYTOCHROME OXIDASE; FURUNCULOSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; DNA HYBRIDIZATION;

D O I：

10.7589/0090-3558-28.4.515

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Cytochrome oxidase variants of the bacterial fish pathogen, Aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. Protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. Pathogenicity of aberrant isolates to brook trout (Salvelinus fontinalis) was similar to typical cytochrome oxidase-positive isolates. Colorimetric deoxyribonucleic acid (DNA) hybridization in 96-well microplates yielded homology values greater than 82.5% for typical aberrant A. salmonicida isolates when photobiotinylated DNA for reference A. salmonicida 3.101 was used as a probe. The only variation of these isolates from typical A. salmonicida was a negative cytochrome oxidase reaction.

引用

页码：515 / 520

页数：6

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