IMIDAZOLE-SDS-ZN REVERSE STAINING OF PROTEINS IN GELS CONTAINING OR NOT SDS AND MICROSEQUENCE OF INDIVIDUAL UNMODIFIED ELECTROBLOTTED PROTEINS

被引：93

作者：

ORTIZ, ML

CALERO, M

PATRON, CF

CASTELLANOS, L

MENDEZ, E

机构：

[1] HOSP RAMON & CAJAL,SERV ENDOCRINOL,E-28034 MADRID,SPAIN

[2] CTR GENET ENGN & BIOTECHNOL,HAVANA 6,CUBA

来源：

FEBS LETTERS | 1992年 / 296卷 / 03期

关键词：

IMIDAZOLE; REVERSE STAINING; MICROSEQUENCING; PAGE;

D O I：

10.1016/0014-5793(92)80309-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse staining procedure is described for the detection of proteins in acrylamide and agarose gels with and without SDS. Protein detection occurs a few minutes after electrophoresis. The sensitivity on acrylamide gels is higher than that of Coomassie blue staining either on acrylamide gels or on electrotransferred membranes. Sequencing of protein bands only detected by reverse staining on the gel and not by Coomassie blue is demonstrated.

引用

页码：300 / 304

页数：5

共 14 条

[1]

ADAMS L D, 1990, Applied and Theoretical Electrophoresis, V1, P279

[2]

BRUNETTE WN, 1981, ANAL BIOCHEM, V112, P195

[3] SODIUM DODECYL-SULFATE GEL-ELECTROPHORESIS - STAINING OF POLYPEPTIDES USING HEAVY-METAL SALTS [J].