CONSERVED AMINO-ACID-RESIDUES IN RIBOSOME-INACTIVATING PROTEINS FROM PLANTS

被引：54

作者：

FUNATSU, G

ISLAM, MR

MINAMI, Y

SUNGSIL, K

KIMURA, M

机构：

[1] Laboratory of Protein Chemistry and Engineering, Faculty of Agriculture, Kyushu University, Fukuoka

来源：

BIOCHIMIE | 1991年 / 73卷 / 7-8期

关键词：

RIBOSOME-INACTIVATING PROTEINS; CONSERVED AMINO ACIDS; ACTIVE-SITE; AMINO ACID SEQUENCES;

D O I：

10.1016/0300-9084(91)90160-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The amino acid sequences of eleven RIPs sequenced to date have been compared in the expectation that this would be useful in the location of functionally and/or structurally important sites of these molecules. In addition to several highly conserved hydrophobic amino acids, thirteen absolutely conserved residues have been found in ricin A-chain: Tyr21, Phe24, Arg29, Tyr80, Tyr123, Gly140, Ala165, Glu177, Ala178, Arg180, Glu208, Asn209 and Trp211. The role of these residues as well as of the C-terminal region have been discussed based on the results of chemical and enzymatic modifications, site-directed mutagenesis, and deletion studies.

引用

页码：1157 / 1161

页数：5

共 43 条

[1] THE COMPLETE PRIMARY STRUCTURE OF PROTEIN-SYNTHESIS INHIBITOR-II FROM BARLEY-SEEDS [J].