SUBZERO TEMPERATURE OPERATING BIOSENSOR UTILIZING AN ORGANIC-SOLVENT AND QUINOPROTEIN GLUCOSE-DEHYDROGENASE

被引：26
作者：
SODE, K
NAKASONO, S
TANAKA, M
MATSUNAGA, T
机构：
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1993年 / 42卷 / 02期
关键词：
BIOSENSOR; SUBZERO TEMPERATURE; PQQGDH;
D O I：
10.1002/bit.260420214
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A subzero temperature operating biosensor was constructed using immobilized quinoprotein glucose dehydrogenase (PQQGDH), glassy carbon electrode, soluble electron mediator (ferrocene monocarboxylic acid), and an organic solvent, ethylene glycol, as an antifreezing reagent. Using this biosensor, glucose concentration can be determined even at -7-degrees-C. At this temperature, the response was 20% of that obtained at 20-degrees-C. This is the first study describing a subzero temperature operating biosensor.
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页码：251 / 254
页数：4
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