PHYTOECDYSTEROID ANALYSIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY

被引：9
作者：
MARCO, MP [1 ]
SANCHEZBAEZA, FJ [1 ]
CAMPS, F [1 ]
COLL, J [1 ]
机构：
[1] CSIC,CID,DEPT BIOL ORGAN CHEM,J GIRONA SALGADO 18-26,E-08034 BARCELONA,SPAIN
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 641卷 / 01期
关键词：
D O I：
10.1016/0021-9673(93)83461-Z
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The potential of high-performance liquid chromatography-mass spectrometry with thermospray interface to identify and analyse ecdysteroids has been investigated. The response of eight different ecdysteroids and their acetonide derivatives is studied by positive- and negative-ion detection as well as with single-ion monitoring and scan mass detection modes. The usefulness of this technique for ecdysteroid identification and quantitation is discussed and, finally, the identification of a new phytoecdysteroid isolated from Polypodium vulgare is presented as an application of this technology. A fast and easy procedure for the extraction and purification of phytoecdysteroids is also described.
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页码：81 / 87
页数：7
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