PHYTOECDYSTEROID ANALYSIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY

被引：9

作者：

MARCO, MP ^{[1
]}

SANCHEZBAEZA, FJ ^{[1
]}

CAMPS, F ^{[1
]}

COLL, J ^{[1
]}

机构：

[1] CSIC,CID,DEPT BIOL ORGAN CHEM,J GIRONA SALGADO 18-26,E-08034 BARCELONA,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 641卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)83461-Z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The potential of high-performance liquid chromatography-mass spectrometry with thermospray interface to identify and analyse ecdysteroids has been investigated. The response of eight different ecdysteroids and their acetonide derivatives is studied by positive- and negative-ion detection as well as with single-ion monitoring and scan mass detection modes. The usefulness of this technique for ecdysteroid identification and quantitation is discussed and, finally, the identification of a new phytoecdysteroid isolated from Polypodium vulgare is presented as an application of this technology. A fast and easy procedure for the extraction and purification of phytoecdysteroids is also described.

引用

页码：81 / 87

页数：7

共 14 条

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